Anti-PRIC4/PRICKLE4 antibody (ab119773)
Key features and details
- Rabbit polyclonal to PRIC4/PRICKLE4
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRIC4/PRICKLE4 antibody -
Description
Rabbit polyclonal to PRIC4/PRICKLE4 -
Host species
Rabbit -
Specificity
Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members. -
Tested applications
Suitable for: WB, ICC/IF, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PRIC4/PRICKLE4 (N terminal).
Database link: NP_037529 -
Positive control
- A549 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 37 kDa.
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ICC/IF |
Use a concentration of 20 µg/ml.
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ICC |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 37 kDa. |
ICC/IF
Use a concentration of 20 µg/ml. |
ICC
Use a concentration of 5 µg/ml. |
Target
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Tissue specificity
Expressed in a broad range of normal tissues as well as in hepatocellular carcinoma, breast cancer and prostate cancer tissues. -
Sequence similarities
Belongs to the prickle / espinas / testin family.
Contains 2 LIM zinc-binding domains.
Contains 1 PET domain. - Information by UniProt
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Database links
- Entrez Gene: 29964 Human
- Omim: 611389 Human
- SwissProt: Q2TBC4 Human
- Unigene: 715653 Human
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Alternative names
- C6orf49 antibody
- OEBT antibody
- Overexpressed breast tumor protein antibody
see all
Images
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Immunofluorescence of PRIC4/PRICKLE4 in A549 cells with ab119773 at 20 ug/mL.
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All lanes : Anti-PRIC4/PRICKLE4 antibody (ab119773) at 0.25 µg/ml
Lane 1 : A549 cell lysate without blocking peptide
Lane 2 : A549 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 37 kDa -
Immunocytochemical analysis of A549 cells, staining PRIC4/PRICKLE4 with ab119773 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab119773 has been referenced in 1 publication.
- MacNair L et al. MTHFSD and DDX58 are novel RNA-binding proteins abnormally regulated in amyotrophic lateral sclerosis. Brain 139:86-100 (2016). ICC/IF ; Mouse . PubMed: 26525917