Product nameAnti-PRKX antibody - C-terminal
See all PRKX primary antibodies
DescriptionRabbit polyclonal to PRKX - C-terminal
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide from the C-terminal region of human PKX conjugated to KLH.
- Human lung carcinoma tissue. Jurkat cell lysate. Mouse brain tissue lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesab71619 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab71619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||1/10 - 1/50.|
Tissue specificityHigh levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver.
Involvement in diseaseNote=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
- Information by UniProt
- PKX1 antibody
- PRKX antibody
- PRKX_HUMAN antibody
All lanes : Anti-PRKX antibody - C-terminal (ab71619) at 1/100 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size: 41 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 32 kDa. We are unsure as to the identity of these extra bands.
ab71619 at 1/10 dilution, staining PKX in human lung carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
ab71619 has not yet been referenced specifically in any publications.