Anti-PRMT1 antibody [MAT-B12] (ab7027)
Key features and details
- Mouse monoclonal [MAT-B12] to PRMT1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PRMT1 antibody [MAT-B12]
See all PRMT1 primary antibodies -
Description
Mouse monoclonal [MAT-B12] to PRMT1 -
Host species
Mouse -
Specificity
This Mab reacts with human arginine N-Methyl Transferase -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human PRMT1.
Database link: Q99873 -
Positive control
- Tested on HELA whole cell extract and transfected Cos cells (recombinant protein PRMT1). The results showed good reactivity of the antibody in both cases. ICC/IF - MCF-7 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MAT-B12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein arginine N-methyltransferase family. -
Cellular localization
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 3276 Human
- Omim: 602950 Human
- SwissProt: Q99873 Human
- Unigene: 20521 Human
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Alternative names
- ANM 1 antibody
- ANM1 antibody
- ANM1_HUMAN antibody
see all
Images
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Immunofluorecent analysis of MCF-7 cells labelling PRMT1 with ab7027
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Overlay histogram showing HeLa cells stained with ab7027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7027, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab7027 has been referenced in 18 publications.
- Sung BY et al. Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1. J Clin Invest 132:N/A (2022). PubMed: 35040433
- Wu D et al. CTNNAL1 participates in the regulation of mucus overproduction in HDM-induced asthma mouse model through the YAP-ROCK2 pathway. J Cell Mol Med 26:1656-1671 (2022). PubMed: 35092120
- Hsu JH et al. PRMT1-Mediated Translation Regulation Is a Crucial Vulnerability of Cancer. Cancer Res 77:4613-4625 (2017). PubMed: 28655788
- Wang YC et al. Identification, chromosomal arrangements and expression analyses of the evolutionarily conserved prmt1 gene in chicken in comparison with its vertebrate paralogue prmt8. PLoS One 12:e0185042 (2017). PubMed: 28934323
- Esse R et al. Protein arginine methylation is more prone to inhibition by S-adenosylhomocysteine than DNA methylation in vascular endothelial cells. PLoS One 8:e55483 (2013). WB ; Human . PubMed: 23408989