Anti-PSD95 antibody [6G6-1C9] (ab2723)

Reviews (12)Q&A (3)References (77)

Overview

  • Product name
    Anti-PSD95 antibody [6G6-1C9]
    See all PSD95 primary antibodies
  • Description
    Mouse monoclonal [6G6-1C9] to PSD95
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Zebrafish
    Predicted to work with: Cow
  • Immunogen

    Recombinant full length protein corresponding to PSD95.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in rat and mouse forebrain and hippocampus tissue lysates.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

Properties

Applications

Our Abpromise guarantee covers the use of ab2723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/3000. PubMed: 22539981
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Domain
    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • Post-translational
    modifications
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • Cellular localization
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Images

  • Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)Image courtesy of an AbReview submitted by Grant Corbett
    Lanes 1-6 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution
    Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution (18 hours at 4°C)

    Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury

    ab2723 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS- 0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab2723 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 (which stains the axons and dendrytic spines) can be observed in red (Alexa fluro 568 secondary). Synaptophysin is observable in green (alexa 488 secondary) and colocalizes with PSD95 in dendritic spines. Nuclei are stained in blue with 1.43µM Hoechst.

    See Abreview

  • Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    All lanes : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 5 µg/ml

    Lane 1 : Hippocampus (Rat) Tissue Lysate
    Lane 2 : Forebrain (Rat) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an Abreview submitted by Ryan MacDonald

    ab2723 staining PSD95 in Zebrafish retina (outer plexiform layer) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Sodium citrate antigen retrieval was used. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an Abreview submitted by Dr Farida Korobova

    ab2723 at 1/200 staining rat primary hippocampal neurons by ICC/IF. The cells were paraformaldehyde fixed and then permeabilized with 0.1% Triton X-100 before being stained with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat polyclonal antibody was used as the secondary.

    The antibody shows numerous puncta along the shafts.

    See Abreview

  • Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an anonymous Abreview
    All lanes : PSD95 antibody (ab2723) at 1/2000 dilution (in blocking buffer for 16 hours at 4°C) + whole tissue lysate of Mouse hippocampus at 10µg

    Secondary
    An HRP-conjugated Horse anti-mouse IgG polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions

    Exposure time : 30 seconds

    Blocking Step : 5% Milk for 1 hour at 25°C

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, France
    IHC-FoFr staining of PSD95 on rat hippocampus section using ab2723 (1:3000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

References

This product has been referenced in:
  • Woodhouse A  et al. Repeat propofol anesthesia does not exacerbate plaque deposition or synapse loss in APP/PS1 Alzheimer's disease mice. BMC Anesthesiol 18:47 (2018). Read more (PubMed: 29699479) »
  • Arulsamy A  et al. Evaluation of early chronic functional outcomes and their relationship to pre-frontal cortex and hippocampal pathology following moderate-severe traumatic brain injury. Behav Brain Res 348:127-138 (2018). WB . Read more (PubMed: 29653127) »
See all 77 Publications for this product

Publishing research using ab2723? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 15 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-PSD95 antibody [6G6-1C9]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary hippocampal neurons)
Permeabilization
Yes - 0.2% TritonX 100
Specification
primary hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Dr. Bhavin Shah

Verified customer

Submitted Apr 09 2018

Immunohistochemistry (Frozen sections) abreview for Anti-PSD95 antibody [6G6-1C9]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (retina, outer plexiform layer)
Specification
retina, outer plexiform layer
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Read More

Dr. Ryan Macdonald

Verified customer

Submitted Oct 02 2014

Western blot abreview for Anti-PSD95 [6G6-1C9] antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% in TGS)
Sample
Mouse Tissue lysate - whole (Hippocampus and Liver)
Specification
Hippocampus and Liver
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Read More

Grant Corbett

Verified customer

Submitted Jul 17 2014

Immunoprecipitation abreview for Anti-PSD95 antibody [6G6-1C9]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Mouse Tissue lysate - whole (mouse brain tissue)
Specification
mouse brain tissue
Total protein in input
200 µg
Read More

Abcam user community

Verified customer

Submitted Sep 19 2013

Western blot abreview for Anti-PSD95 antibody [6G6-1C9]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse hippocampus and cerebellum)
Specification
mouse hippocampus and cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Sep 19 2013

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-PSD95 antibody [6G6-1C9]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Read More

Dr. Sophie Pezet

Verified customer

Submitted Jul 04 2012

Question

Will PSD95 expression be altered if toxicity is increased in the cells?

Read More
Answer

We do not know how PSD95 expression would be affected if toxicity is increased in cells. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.

Read More

Question

Inquiry: We are looking for a loading control for our western blots that is at a minimum 75kDa in size (preferably over 100kDa). Is there anything you could recommend? Our cell type is mouse neurons.

Read More
Answer

Thank you for contacting Abcam.

In mouse neurons an alternative to the smaller housekeeping proteins such as beta-actin, or GAPDH commonly used as loading control would be to use synaptic markers such as PSD95 or Piccolo.

In western blot I would recommend use of anti-PSD95 antibodieshttps://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.htmlorhttps://www.abcam.com/PSD95-antibody-6G6-1C9-ab2723.htmlwhich will give you band above your threshold size at 95kDA.

Should you want bands with an even higher molecular weight Piccolo has a predicted size at 540kDA, our producthttps://www.abcam.com/Piccolo-antibody-Synaptic-Marker-ab20664.htmlis, like the above PSD95 antibodies, guaranteed to work on mouse samples in Western blot.



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

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Immunocytochemistry/ Immunofluorescence abreview for Anti-PSD95 antibody [6G6-1C9]

 Excellent
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Hippocampal neurons)
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Specification
Rat Hippocampal neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 12 2012

Question


I would appreciate if you could tell me which is the epitope of PSD95
recognized by the monoclonal antibody [6G6-1C9] to PSD95 ab2723. The
reason why I am asking this information is because I am planning to
perform pull-down and co-ip experiments using several PSD95 deletion
mutants. I am concerned that some of them could not be recognized by the
antibody simply because I have deleted the epitope.

Read More
Answer

Thank you for contacting us.

To our knowledge, the epitope this clone recognizes has not been mapped. The immunogen was full-length rat PSD95.

If you do decide to try this antibody, we would of course be very interested in the results with your deletion mutants. Please see the page at the following link for more information about our Abreview program:

www.abcam.com/abreviews

Read More

1-10 of 15 Abreviews or Q&A

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