Anti-PSD95 antibody [6G6-1C9] (ab2723)
- Datasheet
- References (78)
- Protocols
Overview
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Product nameAnti-PSD95 antibody [6G6-1C9]
See all PSD95 primary antibodies -
DescriptionMouse monoclonal [6G6-1C9] to PSD95
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, IPmore details
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Species reactivityReacts with: Mouse, Rat, Zebrafish
Predicted to work with: Cow -
Immunogen
Recombinant full length protein corresponding to PSD95.
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Positive control
- In Western Blot, this antibody gave a positive signal in rat and mouse forebrain and hippocampus tissue lysates.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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PurityIgG fraction
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ClonalityMonoclonal
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Clone number6G6-1C9
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/3000. PubMed: 22539981 | |
WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa). | |
IHC-Fr | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. |
Target
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FunctionInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
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Tissue specificityBrain.
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Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
DomainThe PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
Post-translational
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density. -
Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
- Information by UniProt
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Database links
- Entrez Gene: 100137840 Cow
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Entrez Gene: 405796 Zebrafish
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- SwissProt: Q6R005 Zebrafish
- Unigene: 27256 Mouse
see all -
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Images
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Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)Image courtesy of an AbReview submitted by Grant CorbettLanes 1-6 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution
Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution (18 hours at 4°C)
Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury
ab2723 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS- 0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab2723 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 (which stains the axons and dendrytic spines) can be observed in red (Alexa fluro 568 secondary). Synaptophysin is observable in green (alexa 488 secondary) and colocalizes with PSD95 in dendritic spines. Nuclei are stained in blue with 1.43µM Hoechst.
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All lanes : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 5 µg/ml
Lane 1 : Hippocampus (Rat) Tissue Lysate
Lane 2 : Forebrain (Rat) Tissue Lysate
Lane 3 : Hippocampus (Mouse) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an Abreview submitted by Ryan MacDonald
ab2723 staining PSD95 in Zebrafish retina (outer plexiform layer) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Sodium citrate antigen retrieval was used. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an Abreview submitted by Dr Farida Korobova
ab2723 at 1/200 staining rat primary hippocampal neurons by ICC/IF. The cells were paraformaldehyde fixed and then permeabilized with 0.1% Triton X-100 before being stained with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat polyclonal antibody was used as the secondary.
The antibody shows numerous puncta along the shafts.
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Western blot - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an anonymous AbreviewAll lanes : PSD95 antibody (ab2723) at 1/2000 dilution (in blocking buffer for 16 hours at 4°C) + whole tissue lysate of Mouse hippocampus at 10µg
Secondary
An HRP-conjugated Horse anti-mouse IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions
Exposure time : 30 seconds
Blocking Step : 5% Milk for 1 hour at 25°C -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-PSD95 antibody [6G6-1C9] (ab2723)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, FranceIHC-FoFr staining of PSD95 on rat hippocampus section using ab2723 (1:3000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Protocols
References
This product has been referenced in:
- Merino-Serrais P et al. 27-Hydroxycholesterol Induces Aberrant Morphology and Synaptic Dysfunction in Hippocampal Neurons. Cereb Cortex 29:429-446 (2019). Read more (PubMed: 30395175) »
- Woodhouse A et al. Repeat propofol anesthesia does not exacerbate plaque deposition or synapse loss in APP/PS1 Alzheimer's disease mice. BMC Anesthesiol 18:47 (2018). Read more (PubMed: 29699479) »