Recombinant Anti-PSMA antibody [EP3254] (ab79542)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3254] to PSMA
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSMA antibody [EP3254]
See all PSMA primary antibodies -
Description
Rabbit monoclonal [EP3254] to PSMA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PSMA aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- LnCap lysate. LnCap cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3254 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 100-120 kDa (predicted molecular weight: 84 kDa).
For unpurified, use 1/1000. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 100-120 kDa (predicted molecular weight: 84 kDa). For unpurified, use 1/1000. |
Target
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Function
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
Tissue specificity
Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily. -
Domain
The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. -
Post-translational
modificationsThe first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
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Alternative names
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
Images
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Anti-PSMA antibody [EP3254] (ab79542) at 1/3000 dilution (purified) + LnCaP cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 84 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-PSMA antibody [EP3254] (ab79542) at 1/5000 dilution (unpurified) + LnCap lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab79542 has not yet been referenced specifically in any publications.