Anti-PTGIS/PGIS antibody (ab23668)
Key features and details
- Rabbit polyclonal to PTGIS/PGIS
- Suitable for: WB, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Sheep, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-PTGIS/PGIS antibody
See all PTGIS/PGIS primary antibodies -
Description
Rabbit polyclonal to PTGIS/PGIS -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Cow, Human -
Immunogen
Synthetic peptide corresponding to Mouse PTGIS/PGIS aa 475-490.
Sequence:PEFDLSRYGFGLMQPE
(Peptide available asab187257) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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Purification notes
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
Notes |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2). -
Tissue specificity
Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282021 Cow
- Entrez Gene: 5740 Human
- Entrez Gene: 19223 Mouse
- Entrez Gene: 25527 Rat
- Omim: 601699 Human
- SwissProt: Q29626 Cow
- SwissProt: Q16647 Human
- SwissProt: O35074 Mouse
see all -
Alternative names
- CYP8 antibody
- CYP8A1 antibody
- Cytochrome P450, family 8, subfamily A, polypeptide 1 antibody
see all
Images
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ab23668 (4µg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Datasheets and documents
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Datasheet download
References (13)
ab23668 has been referenced in 13 publications.
- Bae SJ et al. Identification and analysis of novel endometriosis biomarkers via integrative bioinformatics. Front Endocrinol (Lausanne) 13:942368 (2022). PubMed: 36339397
- Pan XY et al. Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury. Lab Invest 101:1210-1224 (2021). PubMed: 34112940
- Kowalczyk-Zieba I et al. Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling. Animals (Basel) 11:N/A (2021). PubMed: 34573602
- Ipseiz N et al. Tissue-resident macrophages actively suppress IL-1beta release via a reactive prostanoid/IL-10 pathway. EMBO J 39:e103454 (2020). PubMed: 32484988
- Ebmeyer J et al. Pyrrolizidine alkaloid-induced alterations of prostanoid synthesis in human endothelial cells. Chem Biol Interact 298:104-111 (2019). PubMed: 30465738