Anti-PTP1B (phospho S50) antibody (ab88472)
Key features and details
- Rabbit polyclonal to PTP1B (phospho S50)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTP1B (phospho S50) antibody
See all PTP1B primary antibodies -
Description
Rabbit polyclonal to PTP1B (phospho S50) -
Host species
Rabbit -
Specificity
ab88472 reactivity is observed as a weak singlet in control (untreated) cells and is not observed after lambda phosphatase treatment. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human PTP1B (phospho S50).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5770 Human
- Entrez Gene: 24697 Rat
- Omim: 176885 Human
- SwissProt: P18031 Human
- SwissProt: P20417 Rat
- Unigene: 417549 Human
- Unigene: 11317 Rat
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Alternative names
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab88472 has been referenced in 1 publication.
- Geng D et al. Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels. Commun Biol 3:306 (2020). PubMed: 32533049