Product nameAnti-PYGM antibody
See all PYGM primary antibodies
DescriptionRabbit polyclonal to PYGM
SpecificitySamples used to test ab81901 were: muscle and liver homogenates, purified glycogen. ab81901 will recogize glycogen phosphorylase from both liver and muscle. It has been used in ICC on human primary myotubes.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Dog
KLH-conjugated peptide derived from the sequence of human glycogen phosphorylase.
- Rat liver homogenate. Human skeletal muscle homogenate. Muscle and liver homogenates, purified glycogen. Human primary myotubes
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab81901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 97 kDa.|
|IP||Use at an assay dependent concentration.
Use at 5µg/500µg human skeletal muscle homogenate.ab81901 did not immunoprecipitate glycogen phosphorylase from rat liver.
FunctionPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Involvement in diseaseDefects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
Sequence similaritiesBelongs to the glycogen phosphorylase family.
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
- Information by UniProt
- Glycogen phosphorylase antibody
- Glycogen phosphorylase muscle form antibody
- muscle form antibody
All lanes : Anti-PYGM antibody (ab81901) at 1/1000 dilution
Lane 1 : Rat liver homogenate at 50 µg
Lane 2 : Purified rat liver glycogen at 10 µg
Lane 3 : Human skeletal muscle homogenate at 50 µg
Lane 4 : IP from 500 ug Human skeletal muscle homogenate
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 5 minutes
This product has been referenced in:
- Feng YZ et al. Loss of perilipin 2 in cultured myotubes enhances lipolysis and redirects the metabolic energy balance from glucose oxidation towards fatty acid oxidation. J Lipid Res 58:2147-2161 (2017). Read more (PubMed: 28822960) »