Product nameAnti-Pan3 antibody
DescriptionRabbit polyclonal to Pan3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Pan3.
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HepG2 Whole Cell; MOLT4 Whole Cell; Jurkat Whole Cell; Human Liver Tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).|
FunctionFunctions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity.
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
- Information by UniProt
- hPan3 antibody
- PAB dependent poly(A) specific ribonuclease subunit 3 antibody
- PAB-dependent poly(A)-specific ribonuclease subunit 3 antibody
All lanes : Anti-Pan3 antibody (ab88642) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute