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Full length native protein (purified) corresponding to Human Pancreatic alpha amylase.
Our Abpromise guarantee covers the use of ab21156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/8000. Predicted molecular weight: 57 kDa. Strong reaction to 0.15ug and 0.3ug antigen detected. Predicted molecular weight: 57 kDa. Dilution optimised using Chromogenic detection.|
|IHC-P||1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
IHC image of Pancreatic Amylase staining in Human pancreas normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21156, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab21156 staining Pancreatic alpha amylase in mouse pancreas tissue in immunohistochemical analysis (Frozen sections).
Tissue was blocked with 5% BSA for 30 minutes at 21°C. Samples were incubated with primary antibody for 1 hour at 21°C at 1/800 dilution. Renaissance background reducing diluent was used. An Alexa Fluor488-conjugated goat anti-rabbit IgG monoclonal (H+L) was used as secondary antibody at 1/200 dilution.
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