Anti-Parvalbumin antibody (ab11427)
Key features and details
- Rabbit polyclonal to Parvalbumin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Parvalbumin antibody
See all Parvalbumin primary antibodies -
Description
Rabbit polyclonal to Parvalbumin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Rat Parvalbumin. Purified parvalbumin from rat skeletal muscle.
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Positive control
- ICC/IF: U251, HeLa, C6, and rat cordical cells; IHC-P: Human tonsil, cerebellum and skeletal muscle tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.1% Sodium azide
Constituents: 2% BSA, 1.62% Sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (5) |
1/100 - 1/200.
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IHC-P | (5) |
Use a concentration of 1 µg/ml.
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Notes |
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ICC/IF
1/100 - 1/200. |
IHC-P
Use a concentration of 1 µg/ml. |
Target
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Function
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. -
Sequence similarities
Belongs to the parvalbumin family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 5816 Human
- Entrez Gene: 25269 Rat
- Omim: 168890 Human
- SwissProt: P20472 Human
- SwissProt: P02625 Rat
- Unigene: 295449 Human
- Unigene: 2005 Rat
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Alternative names
- D22S749 antibody
- MGC116759 antibody
- Parvalbumin alpha antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Parvalbumin (green) with ab11427 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/Immunofluorescence analysis of C6 (rat glial tumor cell line) cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemcial immunofluorescence analysis of 4% PFA & 0.2% Picric acid fixed rat cordical cells in culture, labelling parvalbumin with ab11427 at a dilution of 1/500 incubated for 12 hours at 4°C in 10mM PBS & 0.03% Triton X diluent blend. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.
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ab11427 staining Parvalbumin in human brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11427 at a 1/1000 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/ rabbit IgG. -
Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (253)
ab11427 has been referenced in 253 publications.
- Augestad IL et al. Normalisation of glucose metabolism by exendin-4 in the chronic phase after stroke promotes functional recovery in male diabetic mice. Br J Pharmacol 179:677-694 (2022). PubMed: 33973246
- Bertero A et al. A Novel Layer 4 Corticofugal Cell Type/Projection Involved in Thalamo-Cortico-Striatal Sensory Processing. J Neurosci 42:1383-1405 (2022). PubMed: 34983816
- Brodovskaya A et al. Distinct Roles of Rodent Thalamus and Corpus Callosum in Seizure Generalization. Ann Neurol 91:682-696 (2022). PubMed: 35226367
- Trnski S et al. The Signature of Moderate Perinatal Hypoxia on Cortical Organization and Behavior: Altered PNN-Parvalbumin Interneuron Connectivity of the Cingulate Circuitries. Front Cell Dev Biol 10:810980 (2022). PubMed: 35295859
- Lukacova K et al. Striatal Injury Induces Overall Brain Alteration at the Pallial, Thalamic, and Cerebellar Levels. Biology (Basel) 11:N/A (2022). PubMed: 35336799