Product nameAnti-Peg3 antibody
DescriptionRabbit polyclonal to Peg3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
- This antibody gave a positive signal in both Mouse and Rat skeletal muscle tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 179 kDa (predicted molecular weight: 179 kDa).|
RelevancePeg3 encodes a C2H2 type zinc finger protein that is implicated in regulating core body temperature, feeding behavior and obesity in mice. The Peg3 gene transcript encodes a zinc finger protein of 1,572 amino acids. Peg3 is expressed in early somites, branchial arches, and other mesodermal tissues, as well as in the hypothalamus. PEG3 induces apoptosis in cooperation with SIAH1A and acts as a mediator between TP53/p53 and BAX in a neuronal death pathway that is activated by DNA damage. PEG3 acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. PEG3 also possesses a tumor suppressing activity in glioma cells. GENE FUNCTION Kim et al. (1997) reported that the PEG3 gene, like other genes within the region on 19q to which it maps, also encodes a Kruppel-type ZNF protein, but one that is distinguished from other ZNF gene products by the fact that it carries 2 novel proline-rich motifs. The mouse and partial human PEG3 gene sequences showed a high level of conservation, despite that fact that 1 of the 2 proline-rich repeats is absent from the human gene. Kim et al. (1997) found that the human gene is expressed at highest levels in ovary and placenta; mouse Peg3, by contrast, is transcribed at highest levels in the adult brain.
- Kruppel-type zinc finger protein antibody
- paternally expressed 3 antibody
- Paternally expressed gene 3 antibody
All lanes : Anti-Peg3 antibody (ab99252) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 179 kDa
Observed band size: 179 kDa
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This product has been referenced in:
- Frey WD et al. Oxytocin receptor is regulated by Peg3. PLoS One 13:e0202476 (2018). Read more (PubMed: 30106994) »
- Ye A et al. PEG3 control on the mammalian MSL complex. PLoS One 12:e0178363 (2017). ChIP . Read more (PubMed: 28609438) »