Anti-Peptide Histidine Methionine antibody [3] (ab36381)
Key features and details
- Mouse monoclonal [3] to Peptide Histidine Methionine
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Peptide Histidine Methionine antibody [3] -
Description
Mouse monoclonal [3] to Peptide Histidine Methionine -
Host species
Mouse -
Specificity
This antibody binds free Peptide Histidine Methionine in solution. It cross reacts with PACAP (approximately 12%, incomplete, non parallel inhibition). It does not cross react with GLP1, GLP2 VIP or glucagon. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic human Peptide Histidine Methionine coupled to carrier.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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For glutaraldehyde linked Peptide Histidine Methionine-ovalbumin (molar ratio 4:1) coated at 1 µg/mL the dilution is 1/30,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Peptide Histidine Methionine (PHM) is a 27 amino acid vasodilator peptide which shares 50% amino acid homology with vasoactive intestinal peptide (VIP) and which is encoded as part of the pro VIP molecule. Accordingly, all pro VIP derived neuropeptides are frequently colocalized, but appear to exert different physiological effects. The VIP/PHM prohormone is produced by neurons in the central and peripheral nervous systems, often together with acetylcholine. PHM belongs to the glucagon secretin family of peptide hormones and like VIP is a vasodilator and stimulator of intestinal fluid secretion. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 7432 Human
- Omim: 192320 Human
- SwissProt: P01282 Human
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Alternative names
- Intestinal peptide PHM27 antibody
- Peptide histidine methioninamide 27 antibody
- Vasoactive intestinal polypeptide antibody
- VIP antibody
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab36381 has not yet been referenced specifically in any publications.