Recombinant
RabMAb

Recombinant Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725)

Overview

  • Product name
    Anti-Phosphomevalonate kinase antibody [EPR15029]
    See all Phosphomevalonate kinase primary antibodies
  • Description
    Rabbit monoclonal [EPR15029] to Phosphomevalonate kinase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Phosphomevalonate kinase aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15126

  • Positive control
    • WB: HEK293 and HeLa whole cell lysates; Human fetal liver, fetal brain, fetal heart and fetal kidney lysates. ICC/IF: HeLa cells.
  • General notes

     

     

    Previously labelled as PMVK.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/300.

Target

  • Tissue specificity
    Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung.
  • Pathway
    Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPMK antibody
    • HUMPMKI antibody
    • OTTHUMP00000035546 antibody
    • Phosphomevalonate kinase antibody
    • PMK antibody
    • PMK PEN antibody
    • PMKA antibody
    • PMKase antibody
    • PMKI antibody
    • PMKPEN antibody
    • PMVK antibody
    • PMVK_HUMAN antibody
    see all

Images

  • All lanes : Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725) at 1/1000 dilution

    Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab199725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199725.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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