Product nameAnti-Piwi antibody
DescriptionRabbit polyclonal to Piwi
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Drosophila melanogaster
Synthetic peptide derived from residues 350 - 450 of Drosophila melanogaster Piwi.
- This antibody gave a positive signal in Drosophila embryo nuclear extract
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab5207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePiwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos.
- P-element induced wimpy testis antibody
- Piwi antibody
Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
Drosophila embryo lysate, 8% TRIS SDS-PAGE.Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli
The arrow indicates Piwi at a predicted MW of 90 kDa.
Pre-immune sera was used as a control and showed no equivalent band (data not shown).
This product has been referenced in:
- Jeffery WR Distal Regeneration Involves the Age Dependent Activity of Branchial Sac Stem Cells in the Ascidian Ciona intestinalis. Regeneration (Oxf) 2:1-18 (2015). Read more (PubMed: 25893097) »
- Patil VS et al. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol 12:61 (2014). WB ; Drosophila melanogaster . Read more (PubMed: 25287931) »