Recombinant Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free (ab232406)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP870Y] to PAEP/Glycodelin - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free
See all PAEP/Glycodelin primary antibodies -
Description
Rabbit monoclonal [EP870Y] to PAEP/Glycodelin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab232406 is the carrier-free version of ab53289.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP870Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
Target
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Function
This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Post-translational
modificationsAt least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5047 Human
- Omim: 173310 Human
- SwissProt: P09466 Human
- Unigene: 532325 Human
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Alternative names
- Alpha uterine protein antibody
- gD antibody
- GdA antibody
see all
Images
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All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 0.3 µg/ml (purified)
Lane 1 : Human placenta tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using ab53289, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : TPA-treated K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaThis data was developed using ab53289, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232406 has not yet been referenced specifically in any publications.