Recombinant Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP870Y] to PAEP/Glycodelin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PAEP/Glycodelin antibody [EP870Y]
See all PAEP/Glycodelin primary antibodies -
Description
Rabbit monoclonal [EP870Y] to PAEP/Glycodelin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PAEP/Glycodelin aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: P09466 -
Positive control
- WB: TPA-treated K562 cell lysate. Human placenta, kidney, and brain tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP870Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
-
Function
This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Post-translational
modificationsAt least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 5047 Human
- Omim: 173310 Human
- SwissProt: P09466 Human
- Unigene: 532325 Human
-
Alternative names
- Alpha uterine protein antibody
- gD antibody
- GdA antibody
see all
Images
-
All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 0.3 µg/ml (purified)
Lane 1 : Human placenta tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
All lanes : Anti-PAEP/Glycodelin antibody [EP870Y] (ab53289) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : TPA-treated K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab53289 has been referenced in 4 publications.
- Cindrova-Davies T et al. Menstrual flow as a non-invasive source of endometrial organoids. Commun Biol 4:651 (2021). PubMed: 34140633
- Turco MY et al. Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol 19:568-577 (2017). WB ; Human . PubMed: 28394884
- Uysal S et al. Correlation of endometrial glycodelin expression and pregnancy outcome in cases with polycystic ovary syndrome treated with clomiphene citrate plus metformin: a controlled study. Obstet Gynecol Int 2015:278591 (2015). PubMed: 25815012
- Palomino WA et al. A single midcycle dose of levonorgestrel similar to emergency contraceptive does not alter the expression of the L-selectin ligand or molecular markers of endometrial receptivity. Fertil Steril 94:1589-94 (2010). PubMed: 19909947