Recombinant
RabMAb

Anti-Plectin antibody [E398P] (ab32528)

Overview

  • Product name
    Anti-Plectin antibody [E398P]
    See all Plectin primary antibodies
  • Description
    Rabbit monoclonal [E398P] to Plectin
  • Host species
    Rabbit
  • Specificity
    ab32528 recognises plectin, a 500-kDa intermediate filament binding protein.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Plectin (C terminal). The exact sequence is proprietary.

  • Positive control
    • Jurkat cell lysate, human skeletal muscle
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    E398P
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 500 kDa (predicted molecular weight: 531 kDa).
IHC-P 1/250.
IP 1/40.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
    • Tissue specificity
      Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
    • Involvement in disease
      Epidermolysis bullosa simplex with pyloric atresia
      Epidermolysis bullosa simplex, with muscular dystrophy
      Epidermolysis bullosa simplex, Ogna type
      Limb-girdle muscular dystrophy 2Q
    • Sequence similarities
      Belongs to the plakin or cytolinker family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 33 plectin repeats.
      Contains 4 spectrin repeats.
    • Domain
      The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
    • Post-translational
      modifications
      Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • EBS1 antibody
      • EBSO antibody
      • HD1 antibody
      • Hemidesmosomal protein 1 antibody
      • PCN antibody
      • pleC antibody
      • PLEC_HUMAN antibody
      • PLEC1 antibody
      • PLEC1b antibody
      • Plectin 1 antibody
      • Plectin 1 intermediate filament binding protein 500kDa antibody
      • Plectin 6 antibody
      • Plectin antibody
      • Plectin-1 antibody
      • PLTN antibody
      see all

    Images

    • Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250
    • Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI

    References

    This product has been referenced in:
    • Kamal AHM  et al. Inflammatory Proteomic Network Analysis of Statin-treated and Lipopolysaccharide-activated Macrophages. Sci Rep 8:164 (2018). WB ; Mouse . Read more (PubMed: 29317699) »
    • Chen X  et al. Plectin-1 Targeted Dual-modality Nanoparticles for Pancreatic Cancer Imaging. EBioMedicine 30:129-137 (2018). WB ; Human . Read more (PubMed: 29574092) »

    See all 20 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Gallbladder)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
    Permeabilization
    No
    Specification
    Gallbladder
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2018

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit monoclonal [E398P] to Plectin (ab32528) is yet to be tested for use by immunocytochemistry and therefore we cannot guarantee it for thi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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