Product nameAnti-Plunc antibody [EPR8381]
See all Plunc primary antibodies
DescriptionRabbit monoclonal [EPR8381] to Plunc
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Plunc aa 150-250. The exact sequence is proprietary.
- A549, PC3, Jurkat, 293T, Human lung squamous cell cancer and Human fetal lung lysates; Jurkat cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.90 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceMay be involved in the airway inflammatory response after exposure to irritants. May be associated with tumor progression. May play a role in innate immune responses of the upper airways.
Cellular localizationSecreted. Note: Found in the nasal mucus. Apical side of airway epithelial cells. Detected in nasal mucus
- BPIFA1 antibody
- bA49G10.5 antibody
- LPLUNC3 antibody
All lanes : Anti-Plunc antibody [EPR8381] (ab131163) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : PC3 cell lysate
Lane 3 : Human lung squamous cell cancer lysate
Lane 4 : Human fetal lung lysate
Lane 5 : Jurkat cell lysate
Lane 6 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of Jurkat cells labelling Plunc (red) or a rabbit IgG negative control (green) with ab131163 antibody at 1/10 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131163 has not yet been referenced specifically in any publications.