Anti-Plzf antibody (ab39354)
Key features and details
- Rabbit polyclonal to Plzf
- Suitable for: WB
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
-
Product name
Anti-Plzf antibody
See all Plzf primary antibodies -
Description
Rabbit polyclonal to Plzf -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human Plzf aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab39353) -
Positive control
- This antibody gave a positive signal in HL60 and Ramos whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353). |
Target
-
Function
Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Skeletal defects, genital hypoplasia, and mental retardation
A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 9 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 534401 Cow
- Entrez Gene: 7704 Human
- Entrez Gene: 235320 Mouse
- Entrez Gene: 353227 Rat
- Entrez Gene: 323269 Zebrafish
- Omim: 176797 Human
- SwissProt: Q05516 Human
- SwissProt: Q802Y8 Zebrafish
see all -
Alternative names
- AI467657 antibody
- lu antibody
- PLZF antibody
see all
Images
-
All lanes : Anti-Plzf antibody (ab39354) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes -
All lanes : Anti-Plzf antibody (ab39354) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab39354 has been referenced in 16 publications.
- Seth A et al. Gene dosage changes in KCTD13 result in penile and testicular anomalies via diminished androgen receptor function. FASEB J 36:e22567 (2022). PubMed: 36196997
- Bode MF et al. Cell type-specific roles of PAR1 in Coxsackievirus B3 infection. Sci Rep 11:14264 (2021). PubMed: 34253819
- Li SH et al. Metabotropic Glutamate Receptor 2/3 Activation Improves Motor Performance and Reduces Pathology in Heterozygous zQ175 Huntington Disease Mice. J Pharmacol Exp Ther 379:74-84 (2021). PubMed: 34330748
- Guo Y et al. Suppressive role of E3 ubiquitin ligase FBW7 in type I diabetes in non-obese diabetic mice through mediation of ubiquitination of EZH2. Cell Death Discov 7:361 (2021). PubMed: 34802056
- Lei JH et al. Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway. World J Stem Cells 12:500-513 (2020). PubMed: 32742567