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Do you have any apparent sizing for 4-12% Bis-Tris? I've used it on a NuPAGE Novex 4-12% Bis-Tris Protein Gel with MOPS running buffer.
Asked on Jul 24 2015
The apparent sizes will be the same as the ones in the second gel on the online datasheet, which is a 10% Bis-Tris gel, MOPS running buffer. The distribution will be different, with a little more separation of the higher molecular weight proteins at the 4% end of your gel compared to the 10% gel on the datasheet.
Tom RuyleAbcam Scientific Support
Answered on Jul 24 2015