Anti-Profilin 1 antibody - Carboxyterminal end (ab50667)
Key features and details
- Rabbit polyclonal to Profilin 1 - Carboxyterminal end
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Profilin 1 antibody - Carboxyterminal end
See all Profilin 1 primary antibodies -
Description
Rabbit polyclonal to Profilin 1 - Carboxyterminal end -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide:
CYEMASHLRRSQY
conjugated to KLH, corresponding to C terminal amino acids 128-140 of Human Profilin 1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
Target
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Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 18643 Mouse
- SwissProt: P62962 Mouse
- Unigene: 2647 Mouse
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Alternative names
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
see all
Images
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Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.
Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.
Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab50667 has been referenced in 13 publications.
- Drazic A et al. The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1. J Mol Biol 434:167397 (2022). PubMed: 34896361
- Zi J et al. PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK. Cells 11:N/A (2022). PubMed: 36291059
- Nejedlá M et al. The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Life Sci Alliance 4:N/A (2021). PubMed: 33184056
- Nekouei M et al. Mutant Profilin1 Aggregation in Amyotrophic Lateral Sclerosis: An in Vivo Biochemical Analysis. Basic Clin Neurosci 12:213-222 (2021). PubMed: 34925718
- Rebowski G et al. Mechanism of actin N-terminal acetylation. Sci Adv 6:eaay8793 (2020). PubMed: 32284999