Recombinant Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2804Y] to Prohibitin - Mitochondrial Marker
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker
See all Prohibitin primary antibodies -
Description
Rabbit monoclonal [EP2804Y] to Prohibitin - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Prohibitin aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: MCF-7, PC-12, A431 and Ramos cell lysate; IHC-P: human hepatocellular carcinoma tissue, mouse kidney tissue, mouse endometrium tissue, mouse liver tissue, rat liver tissue and rat stomach tissue; ICC/IF: Hela and Neuro-2a cells. IP: MCF7
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2804Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 30 kDa.
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IP |
1/110.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 30 kDa. |
IP
1/110. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. -
Tissue specificity
Widely expressed in different tissues. -
Sequence similarities
Belongs to the prohibitin family. -
Developmental stage
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 5245 Human
- Entrez Gene: 18673 Mouse
- Entrez Gene: 25344 Rat
- Omim: 176705 Human
- SwissProt: P35232 Human
- SwissProt: P67778 Mouse
- SwissProt: P67779 Rat
- Unigene: 514303 Human
see all -
Alternative names
- Epididymis luminal protein 215 antibody
- Epididymis secretory sperm binding protein Li 54e antibody
- HEL 215 antibody
see all
Images
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All lanes : Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771) at 1/1000 dilution
Lane 1 : MCF-7 cell lysates
Lane 2 : PC-12 cell lysates
Lane 3 : A431 cell lysates
Lane 4 : Ramos cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Prohibitin with ab75771 at 1/250.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Prohibitin with ab75771 at 1/100. A HRP/AP polymerized secondary antibody was used.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab75771 (purified) at 1/110 dilution (20 µg/ml) immunoprecipitating Prohibitin in MCF7 whole cell lysate .
Lane 1 (input): MCF7(Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab75771 & MCF7 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75771 in MCF7 whole cell lysate
For western blotting, ab75771 at 1/500 dilution (4.3 µg/ml) VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse endometrium tissue labelling Prohibitin with ab75771 at 1/250.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Prohibitin with ab75771 at 1/250.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Prohibitin with ab75771 at 1/250.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling Prohibitin with ab75771 at 1/250.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab75771 has been referenced in 13 publications.
- Uysal-Onganer P et al. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. Int J Mol Sci 22:N/A (2021). PubMed: 33573274
- Wild M et al. The Artemisinin-Derived Autofluorescent Compound BG95 Exerts Strong Anticytomegaloviral Activity Based on a Mitochondrial Targeting Mechanism. Int J Mol Sci 21:N/A (2020). PubMed: 32759737
- Uysal-Onganer P et al. Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines. Int J Mol Sci 21:N/A (2020). PubMed: 32098295
- Li Y et al. Astragaloside IV reduces neuronal apoptosis and parthanatos in ischemic injury by preserving mitochondrial hexokinase-II. Free Radic Biol Med 131:251-263 (2019). PubMed: 30502455
- Kosgodage US et al. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Transl Oncol 12:513-522 (2019). PubMed: 30597288