The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
Belongs to the G-protein coupled receptor 1 family.