Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532)


  • Product name
    Anti-Proteasome 20S alpha 3 antibody [EPR5455]
    See all Proteasome 20S alpha 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5455] to Proteasome 20S alpha 3
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome 20S alpha 3 aa 50-150. The exact sequence is proprietary.
    Database link: P25788

  • Positive control
    • A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab109532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
    • Sequence similarities
      Belongs to the peptidase T1A family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HC8 antibody
      • Macropain subunit C8 antibody
      • MGC12306 antibody
      • MGC32631 antibody
      • Multicatalytic endopeptidase complex subunit C8 antibody
      • Proteasome (prosome macropain) subunit alpha type 3 antibody
      • Proteasome alpha 3 subunit antibody
      • Proteasome component C8 antibody
      • Proteasome subunit alpha type 3 antibody
      • Proteasome subunit alpha type-3 antibody
      • Proteasome subunit C8 antibody
      • PSA3_HUMAN antibody
      • PSC8 antibody
      • psmA3 antibody
      see all


    • All lanes : Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in HeLa cells by Immunofluorescence.


    This product has been referenced in:
    • Cirigliano SM  et al. The synthetic peptide CIGB-300 modulates CK2-dependent signaling pathways affecting the survival and chemoresistance of non-small cell lung cancer cell lines. Cancer Cell Int 17:42 (2017). ICC/IF ; Human . Read more (PubMed: 28373828) »
    • Moritz KE  et al. The role of the immunoproteasome in interferon-?-mediated microglial activation. Sci Rep 7:9365 (2017). Read more (PubMed: 28839214) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    We do not have any data suggesting that the antibody has higher affinity for nuclear PSMA3 than for cytoplasmic PSMA3. The differences in staining intensity may be due to unequal distribution of the protein (more PSMA3 in the nucleus than in the cytopl...

    Read More


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