Recombinant Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10177] to Proteasome maturation protein
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Proteasome maturation protein antibody [EPR10177]
See all Proteasome maturation protein primary antibodies -
Description
Rabbit monoclonal [EPR10177] to Proteasome maturation protein -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Proteasome maturation protein (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y244 -
Positive control
- HeLa, SW480, HL60 and Jurkat cell lysates; Human skin tissue; Permeabilized Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10177 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
IP |
1/10 - 1/100.
|
Notes |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
-
Function
Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum. -
Tissue specificity
Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer. -
Involvement in disease
Defects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) [MIM:601952]. KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer. -
Sequence similarities
Belongs to the POMP/UMP1 family. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Microsome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51371 Human
- Omim: 613386 Human
- SwissProt: Q9Y244 Human
- Unigene: 268742 Human
-
Alternative names
- 2510048O06Rik antibody
- C13orf12 antibody
- Chromosome 13 open reading frame 12 antibody
see all
Images
-
All lanes : Anti-Proteasome maturation protein antibody [EPR10177] (ab170865) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa -
Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red)or a rabbit IgG (negative) (green).
-
Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab170865 has been referenced in 2 publications.
- Schneider K et al. Protein Stability Buffers the Cost of Translation Attenuation following eIF2a Phosphorylation. Cell Rep 32:108154 (2020). PubMed: 32937139
- Rousseau A & Bertolotti A An evolutionarily conserved pathway controls proteasome homeostasis. Nature 536:184-9 (2016). WB . PubMed: 27462806