Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)
Key features and details
- Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies -
Description
Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72) -
Host species
Rabbit -
Specificity
ab27850 recognises Inhibitor-2. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Protein phosphatase 1 inhibitor subunit 2 (phospho T72).
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Positive control
- ICC/IF: A431 cells treated with Nocodazole and HeLa cells; WB: HCT116, HT 29, HEL 92.1.7 and HeLa whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA
1.0 mg/mL BSA (IgG, protease free), Dulbecco's PBS [without magnesium and calcium ions] -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 23 kDa.
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ICC/IF |
1/250.
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Notes |
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WB
1/1000. Predicted molecular weight: 23 kDa. |
ICC/IF
1/250. |
Target
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Function
Inhibitor of protein-phosphatase 1. -
Sequence similarities
Belongs to the protein phosphatase inhibitor 2 family. -
Post-translational
modificationsPhosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex. - Information by UniProt
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Database links
- Entrez Gene: 5504 Human
- Omim: 601792 Human
- SwissProt: P41236 Human
- Unigene: 535731 Human
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Alternative names
- IPP 2 antibody
- IPP-2 antibody
- IPP2 antibody
see all
Images
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All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1 : HCT 116 whole cell lysate
Lane 2 : HT 29 whole cell lysate
Lane 3 : Hel 92.1.7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Secondary Antibody at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescent analysis of A431 cells treated with Nocodazole for 24 hours, staining Protein phosphatase 1 inhibitor subunit 2 at 1/250 dilution, followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X 100 for ten minutes, and blocked with 1% BSA for one hour at room temperature. Nuclei were counterstained with DAPI (blue). F-actin was stained with Rhodamine Phalloidin (red).
Negative controls are untreated A431 cells (e) and a secondary only antibody control (f).
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Immunocyutochemistry/Immunofluroescence analysis of HeLa cells staining Protein phosphatase 1 inhibitor subunit 2 with ab27850 (green). Cells were grown on a chamber slide and fixed in cold 10% trichloroacetic acid for 10 minutes, rinsed in TBS and permeabilized with 0.1% Triton-X-100 for 15 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab27850 has been referenced in 1 publication.
- Belov L et al. Surface Profiling of Extracellular Vesicles from Plasma or Ascites Fluid Using DotScan Antibody Microarrays. Methods Mol Biol 1619:263-301 (2017). PubMed: 28674892