Overview

  • Product name
    Anti-Pur B antibody
  • Description
    Rabbit polyclonal to Pur B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 262-312 of Human Pur B (NP_150093.1).

  • Positive control
    • Jurkat whole cell lysate (ab7899).

Properties

Applications

Our Abpromise guarantee covers the use of ab130999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs.
    • Tissue specificity
      Expressed in myocardium of heart failure patients.
    • Sequence similarities
      Belongs to the PUR DNA-binding protein family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Pur beta antibody
      • purB antibody
      • PURB_HUMAN antibody
      • PURBETA antibody
      • Purine rich element binding protein B antibody
      • Purine-rich element-binding protein B antibody
      • transcriptional activator protein Pur beta antibody
      • Transcriptional activator protein Pur-beta antibody
      see all

    Images

    • Detection of Pur B by Western Blot of Immunprecipitate.
      ab130999 at 1µg/ml staining Pur B in Jurkat whole cell lysate immunoprecipitated using ab1130999 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 minutes.

    References

    ab130999 has not yet been referenced specifically in any publications.

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