Anti-Pyrophosphatase 1 antibody (ab96099)
Key features and details
- Rabbit polyclonal to Pyrophosphatase 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pyrophosphatase 1 antibody
See all Pyrophosphatase 1 primary antibodies -
Description
Rabbit polyclonal to Pyrophosphatase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Monkey -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 259 of Human Pyrophosphatase 1 (UniProt ID: Q15181).
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Positive control
- 293T, Raji. A431, H1299, HeLa, HepG2, MOLT4 whole cell lysates for WB. HeLa cells for IF. OVCAR3 xenograft for IHC.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the PPase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 280701 Cow
- Entrez Gene: 5464 Human
- Entrez Gene: 67895 Mouse
- Entrez Gene: 294504 Rat
- Omim: 179030 Human
- SwissProt: P37980 Cow
- SwissProt: Q15181 Human
- SwissProt: Q9D819 Mouse
see all -
Alternative names
- cytosolic inorganic pyrophosphatase antibody
- diphosphate phosphohydrolase antibody
- inorganic diphosphatase antibody
see all
Images
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All lanes : Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution
Lane 1 : H1299 Whole cell lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : HepG2 Whole cell lysate
Lane 4 : MOLT4 Whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab96099 has been referenced in 5 publications.
- Li H et al. Expression of Inorganic Pyrophosphatase (PPA1) Correlates with Poor Prognosis of Epithelial Ovarian Cancer. Tohoku J Exp Med 241:165-173 (2017). PubMed: 28202851
- Jiang P et al. Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. JPEN J Parenter Enteral Nutr N/A:N/A (2016). PubMed: 27503937
- Megger DA et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). PubMed: 23462207
- Yi YJ et al. Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa. PLoS One 7:e34524 (2012). WB, ICC/IF ; Pig . PubMed: 22485177
- Jiang P et al. Antibiotics increase gut metabolism and antioxidant proteins and decrease acute phase response and necrotizing enterocolitis in preterm neonates. PLoS One 7:e44929 (2012). WB . PubMed: 23028687