Product nameAnti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ]
See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
DescriptionMouse monoclonal [17A5E2H8] to Pyruvate Dehydrogenase E1 beta subunit
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Porcine Pyruvate Dehydrogenase E1 beta subunit protein.
- Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 whole cell lysate (ab7900). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesab110331 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab110331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.|
FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Involvement in diseaseDefects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
Cellular localizationMitochondrion matrix.
- Information by UniProt
- DKFZp564K0164 antibody
- mitochondrial antibody
- ODPB_HUMAN antibody
IHC image of Pyruvate Dehydrogenase E1 beta subunit staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
Lane 5 : HepG2 cell lysate at 20 µg/ml
Predicted band size: 39 kDa
This product has been referenced in:
- Qi X et al. Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis. Reprod Biomed Online 29:761-70 (2014). Read more (PubMed: 25444511) »
- Halim ND et al. Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. Glia 58:1168-76 (2010). Read more (PubMed: 20544852) »