Overview

  • Product name
    Anti-QKI antibody [EPR7305(B)]
    See all QKI primary antibodies
  • Description
    Rabbit monoclonal [EPR7305(B)] to QKI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human QKI. The exact sequence is proprietary.
    Database link: Q96PU8

  • Positive control
    • K562, PC12, HeLa and Neuro 2a cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
    • Tissue specificity
      Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
    • Sequence similarities
      Contains 1 KH domain.
    • Domain
      The KH domain and the Qua2 region are involved in RNA binding.
    • Post-translational
      modifications
      Methylated by PRMT1.
      Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
    • Cellular localization
      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586I0923 antibody
      • HKQ antibody
      • Homolog of mouse quaking QKI KH domain RNA binding protein antibody
      • Hqk antibody
      • HQK1 antibody
      • HqkI antibody
      • OTTHUMP00000017581 antibody
      • OTTHUMP00000017582 antibody
      • OTTHUMP00000017583 antibody
      • Protein quaking antibody
      • QK antibody
      • QK1 antibody
      • QK3 antibody
      • QKI antibody
      • QKI_HUMAN antibody
      • QKI1 antibody
      • Quaking homolog antibody
      • Quaking homolog KH domain RNA binding antibody
      • Quaking homolog KH domain RNA binding mouse antibody
      • Quaking isoform 1 antibody
      • Quaking protein antibody
      • RNA binding protein HQK antibody
      see all

    Images

    • All lanes : Anti-QKI antibody [EPR7305(B)] (ab126791) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Neuro 2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    References

    ab126791 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126791.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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