Rhodamine Anti-R Phycoerythrin/rpeA antibody (ab34717)
Key features and details
- Rhodamine Goat polyclonal to R Phycoerythrin/rpeA
- Suitable for: Immunomicroscopy, Flow Cyt, WB
- Conjugation: Rhodamine. Ex: 550nm, Em: 570nm
- Isotype: IgG
Overview
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Product name
Rhodamine Anti-R Phycoerythrin/rpeA antibody
See all R Phycoerythrin/rpeA primary antibodies -
Description
Rhodamine Goat polyclonal to R Phycoerythrin/rpeA -
Host species
Goat -
Conjugation
Rhodamine. Ex: 550nm, Em: 570nm -
Specificity
This antibody recognizes R Phycoerythrin/rpeA conjugated IgG. It will also cross react with B Phycoerythrin.
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Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Red Algae -
Immunogen
Full length native protein (purified) corresponding to R Phycoerythrin/rpeA.
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General notes
Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons). Absorption Wavelength: 550 nm. Emission Wavelength: 570 nm. Fluorochrome/Protein Ratio: 4.0 moles TRITC per mole of Goat IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from monospecific antiserun by immunoaffinity chromatography using a R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Immunomicroscopy |
Use at an assay dependent dilution.
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Flow Cyt |
Use at an assay dependent dilution.
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.
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Notes |
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Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. Predicted molecular weight: 240 kDa. |
Target
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Relevance
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. -
Cellular localization
Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome. -
Alternative names
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab34717 has been referenced in 1 publication.
- Fujita T et al. Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3:3171 (2013). PubMed: 24201379