Recombinant Anti-RAIDD antibody [EPR1639] (ab76465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1639] to RAIDD
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAIDD antibody [EPR1639]
See all RAIDD primary antibodies -
Description
Rabbit monoclonal [EPR1639] to RAIDD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human RAIDD aa 100-200. The exact sequence is proprietary.
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Positive control
- Raji, HeLa, K562 and MCF7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1639 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 23 kDa. |
Target
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Function
Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. -
Tissue specificity
Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 death domain. -
Domain
Contains a death domain involved in the binding of RIP protein.
The CARD domain mediates the interaction with caspase-2. - Information by UniProt
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Database links
- Entrez Gene: 8738 Human
- Entrez Gene: 314756 Rat
- Omim: 603454 Human
- SwissProt: P78560 Human
- Unigene: 38533 Human
- Unigene: 591016 Human
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Alternative names
- CASP2 and RIPK1 domain containing adaptor with death domain antibody
- Caspase and RIP adapter with death domain antibody
- Caspase and RIP adaptor with death domain antibody
see all
Images
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All lanes : Anti-RAIDD antibody [EPR1639] (ab76465) at 1/5000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab76465 has been referenced in 2 publications.
- Vigneswara V & Ahmed Z Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2. Cell Death Dis 10:102 (2019). PubMed: 30718480
- Di Donato N et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 99:1117-1129 (2016). PubMed: 27773430