Overview

  • Product name
  • Description
    Mouse polyclonal to RAP55
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human RAP55.
    Database link: AAH16842.1

  • Positive control
    • RAP55 transfected 293T cell lysate. Human placenta tissue lysate.
  • General notes

    Previously labelled as LSM14A. 

Properties

Applications

Our Abpromise guarantee covers the use of ab89038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.

Target

  • Function
    Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs.
  • Sequence similarities
    Belongs to the LSM14 family.
    Contains 1 DFDF domain.
  • Domain
    The LSM14 domain and the RGG repeats are required for accumulation in P-bodies, and the region containing the FDF motif is responsible for cytoplasmic retention.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cytoplasm > P-body. Targeted to stress granules (SGs) in response to cellular stress.
  • Information by UniProt
  • Database links
  • Alternative names
    • AlphaSNBP antibody
    • C19orf13 antibody
    • Chromosome 19 open reading frame 13 antibody
    • DKFZp434D1335 antibody
    • FAM61A antibody
    • Family with sequence similarity 61 member A antibody
    • hRAP55 antibody
    • hRAP55A antibody
    • LS14A_HUMAN antibody
    • LSM14 homolog A (SCD6 S cerevisiae) antibody
    • LSM14 homolog A antibody
    • LSM14A antibody
    • Protein FAM61A antibody
    • Protein LSM14 homolog A antibody
    • Protein SCD6 homolog antibody
    • Putative alpha synuclein binding protein antibody
    • Putative alpha-synuclein-binding protein antibody
    • RAP55 antibody
    • RNA associated protein 55 antibody
    • RNA-associated protein 55A antibody
    • SCD6 homolog A (S cerevisiae) antibody
    see all

Images

  • Anti-RAP55 antibody (ab89038) at 1/500 dilution + human placenta lysate at 50 µg

    Predicted band size: 50 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-RAP55 antibody (ab89038) at 1/500 dilution

    Lane 1 : RAP55 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 50 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

References

ab89038 has not yet been referenced specifically in any publications.

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