Anti-RAP55 antibody (ab89038)
Key features and details
- Mouse polyclonal to RAP55
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAP55 antibody
See all RAP55 primary antibodies -
Description
Mouse polyclonal to RAP55 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RAP55. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: AAH16842.1 -
Positive control
- RAP55 transfected 293T cell lysate. Human placenta tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa. |
Target
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Function
Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs. -
Sequence similarities
Belongs to the LSM14 family.
Contains 1 DFDF domain. -
Domain
The LSM14 domain and the RGG repeats are required for accumulation in P-bodies, and the region containing the FDF motif is responsible for cytoplasmic retention. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cytoplasm > P-body. Targeted to stress granules (SGs) in response to cellular stress. - Information by UniProt
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Database links
- Entrez Gene: 26065 Human
- Omim: 610677 Human
- SwissProt: Q8ND56 Human
- Unigene: 407368 Human
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Alternative names
- AlphaSNBP antibody
- C19orf13 antibody
- Chromosome 19 open reading frame 13 antibody
see all
Images
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Anti-RAP55 antibody (ab89038) at 1/500 dilution + human placenta lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RAP55 antibody (ab89038) at 1/500 dilution
Lane 1 : RAP55 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab89038 has been referenced in 1 publication.
- Zhang L et al. Nonstructural Protein NSs Hampers Cellular Antiviral Response through LSm14A during Severe Fever with Thrombocytopenia Syndrome Virus Infection. J Immunol 207:590-601 (2021). PubMed: 34244294