Recombinant Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RASGRP3 (phospho T133) antibody [EPNCIR148]
See all RASGRP3 primary antibodies -
Description
Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: MouseDoes not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Ramos cell lysates
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General notes
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR148 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa. |
Target
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Function
Guanine nucleotide exchange factor (GEF) for Ras and Rap1. -
Sequence similarities
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain. - Information by UniProt
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Database links
- Entrez Gene: 25780 Human
- Entrez Gene: 240168 Mouse
- Omim: 609531 Human
- SwissProt: Q8IV61 Human
- Unigene: 143674 Human
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Alternative names
- Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
- Grp3 antibody
- GRP3_HUMAN antibody
see all
Images
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All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution
Lane 1 : Ramos cell lysate, untreated
Lane 2 : Ramos cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124823 has been referenced in 3 publications.
- Tang S et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . PubMed: 25118589
- Nagy Z et al. Function of RasGRP3 in the formation and progression of human breast cancer. Mol Cancer 13:96 (2014). PubMed: 24779681
- Song X et al. RasGRPs are targets of the anti-cancer agent ingenol-3-angelate. PLoS One 8:e72331 (2013). WB ; Rat . PubMed: 23991094