For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to RBM12
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 625 and 675 of human RBM12 (NP_690051.1).
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 97 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 3 RRM (RNA recognition motif) domains.
Information by UniProt
RBM 12 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM12 antibody (ab72319)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RBM12 with ab72319 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-RBM12 antibody (ab72319)
All lanes :
Anti-RBM12 antibody (ab72319) at 0.04 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Predicted band size:
Observed band size:
117 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-RBM12 antibody (ab72319)
Detection of Human RBM12 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72319 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"