Overview

  • Product name
  • Description
    Rabbit polyclonal to RILP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cat
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 333-382 (PPPPESKIQS FFGLWYRGKA ESSEDETSSP APSKLGGEEE AQPQSPAPDP) of Human RILP (NP_113618).

  • Positive control
    • Human placenta lysate IF/ICC: MCF7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab128616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Rab effector playing a role in late endocytic transport to degradative compartments. Involved in the regulation of lysosomal morphology and distribution. Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments. Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes.
  • Tissue specificity
    Ubiquitous. Strongly expressed in fetal heart, heart, stomach, spleen, adrenal gland, thyroid gland, salivary gland, fetal liver, liver and lung. Poorly expressed in brain.
  • Sequence similarities
    Contains 1 RILP-like domain.
  • Cellular localization
    Endomembrane system. Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle > phagosome membrane. Associated with late endosomal, lysosomal and phagosomal membranes. The interaction with RAB7A is necessary for its recruitment to phagosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ31193 antibody
    • PP10141 antibody
    • Rab interacting lysosomal protein antibody
    • Rab-interacting lysosomal protein antibody
    • Rilp antibody
    • RILP_HUMAN antibody
    see all

Images

  • ICC/IF image of ab128616 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128616, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RILP antibody (ab128616) at 1 µg/ml + Human placenta lysate

    Predicted band size: 44 kDa



    Gel concentration: 12%

References

This product has been referenced in:
  • Im EJ  et al. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. Nat Commun 10:1387 (2019). Read more (PubMed: 30918259) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab128616.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up