The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 84 kDa).
Use at an assay dependent concentration.
E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF98 antibody (ab4836)This image is courtesy of an abreview by Zachary Yu-Ching Lin
IHC-P image of RNF98 staining with ab4836 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab4836 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated Donkey anti-Goat polyclonal, used at a 1/500 dilution.
Yu-Ching Lin Z et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev BiolN/A:N/A (2015).
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