Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
Key features and details
- Mouse monoclonal [8C3-D12-H10] to RPA70
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-RPA70 antibody [8C3-D12-H10]
See all RPA70 primary antibodies -
Description
Mouse monoclonal [8C3-D12-H10] to RPA70 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human RPA70. Purified recombinant human RPA70 protein fragments expressed in E.coli
Database link: P27694 -
Positive control
- COS7, HT1080, A431, MCF7, K562, HeLa, 293T, 3T3 and C6 cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
8C3-D12-H10 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB | (1) |
1/1000. Predicted molecular weight: 68 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 68 kDa. |
Target
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Function
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
Sequence similarities
Belongs to the replication factor A protein 1 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6117 Human
- Entrez Gene: 68275 Mouse
- Entrez Gene: 287524 Rat
- Omim: 179835 Human
- SwissProt: P27694 Human
- SwissProt: Q8VEE4 Mouse
- Unigene: 461925 Human
- Unigene: 180734 Mouse
see all -
Alternative names
- Dmrpa1 antibody
- Drosophila Replication Protein A antibody
- DRPA antibody
see all
Images
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution
Lane 1 : C6 Cell lysate
Lane 2 : COS7 Cell lysate
Lane 3 : K562 Cell lysate
Lane 4 : HeLa Cell lysate
Predicted band size: 68 kDa -
Immunocytochemistry/Immunofluorscence analysis of HeLa cells fixed with paraformaldehyde, labeling RPA70 with ab176467 at 1/100. Left - RPA70, Middle - nuclear staining, Right - Merge. This image was produced with antibody produced in ascites.
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : C6 cell lysate
Predicted band size: 68 kDaThis image was produced with antibody produced in ascites.
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
Lane 1 : COS7 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : HeLa cell lysate
Predicted band size: 68 kDaThis image was produced with antibody produced in ascites.
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Immunoprecipitation analysis of HeLa cell lysate labeling RPA70 with ab176467. This image was produced with antibody produced in ascites.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab176467 has been referenced in 4 publications.
- Hu C et al. Beta human papillomavirus 8 E6 allows colocalization of non-homologous end joining and homologous recombination repair factors. PLoS Pathog 18:e1010275 (2022). PubMed: 35148356
- Kang JW et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736
- Fowler S et al. LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Sci Rep 8:9536 (2018). PubMed: 29934626
- Ghospurkar PL et al. Phosphorylation and cellular function of the human Rpa2 N-terminus in the budding yeast Saccharomyces cerevisiae. Exp Cell Res 331:183-99 (2015). WB . PubMed: 25499885