Recombinant Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (ab213165)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - BSA and Azide free
- Suitable for: Dot blot, WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free
See all RPS6 primary antibodies -
Description
Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate, untreated or treated with Calyculin A. IHC: Human breast adenocarcinoma tissue. IP: 293T lysate.
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General notes
ab213165 is the carrier-free version of ab80158.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1338(2)Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
Sequence similarities
Belongs to the ribosomal protein S6e family. -
Post-translational
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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Database links
- Entrez Gene: 6194 Human
- Omim: 180460 Human
- SwissProt: P62753 Human
- Unigene: 408073 Human
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Alternative names
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
Images
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This data was developed using ab80158, the same antibody clone in a different buffer formulation.
RPS6 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 µg with ab80158 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: 293T (Human embryonic kidney epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 µg
Lane 2: abab80158 IP in 293T treated with Calyculin A(100nM 30min) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab80158 in 293T treated with Calyculin A(100nM 30min) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Dot Blot analysis of -
Lane 1: Rb (pS235) phospho peptide
Lane 2: Rb (pS236) phospho peptide
Lane 3: Rb (pS235 & pS236) phospho peptide
Lane 4: Rb non-phospho peptide
5% NFDM/TBST was used as the diluting and blocking buffer.
ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).
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ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab213165 has not yet been referenced specifically in any publications.