Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed (ab7131)
Key features and details
- Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Fr
Overview
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Product name
Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Tested applications
Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Frmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Goat IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Tripotassium orthophosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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|
Dot blot |
Use at an assay dependent concentration.
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|
ELISA |
Use at an assay dependent dilution.
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|
IHC-P |
Use at an assay dependent concentration.
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|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent dilution.
|
|
Flow Cyt |
Use at an assay dependent dilution. PubMed: 20838433
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
WB
Use at an assay dependent dilution. |
Flow Cyt
Use at an assay dependent dilution. PubMed: 20838433 |
IHC-Fr
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab7131 has been referenced in 1 publication.
- Fernandez P et al. Strain-transcendent immune response to recombinant Var2CSA DBL5-e domain block P. falciparum adhesion to placenta-derived BeWo cells under flow conditions. PLoS One 5:e12558 (2010). Flow Cyt . PubMed: 20838433