Anti-Rad21 antibody (ab154769)
Key features and details
- Rabbit polyclonal to Rad21
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Rad21 antibody
See all Rad21 primary antibodies -
Description
Rabbit polyclonal to Rad21 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 6-399 of Human Rad21 (UniProt ID: O60216).
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Positive control
- K562, THP1, HL60, NCI-H929, NIH3T3, JC and BCL-1 whole cell lysate; HeLa cells; Human HBL435 and Mouse C2C12 xenograft tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Detects a band of approximately 130 kDa (predicted molecular weight: 72 kDa).
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Detects a band of approximately 130 kDa (predicted molecular weight: 72 kDa). |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. -
Sequence similarities
Belongs to the rad21 family. -
Domain
The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3. -
Post-translational
modificationsCleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 5885 Human
- Entrez Gene: 19357 Mouse
- Omim: 606462 Human
- SwissProt: O60216 Human
- SwissProt: Q61550 Mouse
- Unigene: 81848 Human
- Unigene: 182628 Mouse
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Alternative names
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
see all
Images
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All lanes : Anti-Rad21 antibody (ab154769) at 1/1000 dilution
Lane 1 : K562 whole cell lysate
Lane 2 : THP1 whole cell lysate
Lane 3 : HL60 whole cell lysate
Lane 4 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 72 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
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Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
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All lanes : Anti-Rad21 antibody (ab154769) at 1/3000 dilution
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 72 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab154769 has been referenced in 25 publications.
- Gines MM & Wendt KS A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing. Methods Mol Biol 2458:113-122 (2022). PubMed: 35103965
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136
- Aljahani A et al. Analysis of sub-kilobase chromatin topology reveals nano-scale regulatory interactions with variable dependence on cohesin and CTCF. Nat Commun 13:2139 (2022). PubMed: 35440598
- Robles-Rebollo I et al. Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters. Nat Commun 13:4342 (2022). PubMed: 35896525
- van Schaik T et al. CTCF and cohesin promote focal detachment of DNA from the nuclear lamina. Genome Biol 23:185 (2022). PubMed: 36050765