Anti-RanBP1 antibody (ab70185)
Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RanBP1 antibody
See all RanBP1 primary antibodies -
Description
Rabbit polyclonal to RanBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding a region between residues 1 and 50 of human RanBP1 (NP_002873.1)
-
Positive control
- HeLa whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab70185 was affinity purified using an epitope specific to RanBP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
IP |
Notes |
---|
WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
Sequence similarities
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
-
Database links
- Entrez Gene: 5902 Human
- Omim: 601180 Human
- SwissProt: P43487 Human
- Unigene: 24763 Human
-
Alternative names
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
Images
-
Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml
All lanes : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab70185 has not yet been referenced specifically in any publications.