Anti-RanGAP1 antibody (ab2081)
Key features and details
- Goat polyclonal to RanGAP1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-RanGAP1 antibody
See all RanGAP1 primary antibodies -
Description
Goat polyclonal to RanGAP1 -
Host species
Goat -
Specificity
There is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide: ASEDIAKLAETLAK, corresponding to N terminal amino acids 2-15 of Human RanGAP1.
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Positive control
- 3T3 lysate.
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General notes
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa.
A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874). |
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ICC/IF |
1/500.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa. A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874). |
ICC/IF
1/500. |
Target
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Function
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. -
Tissue specificity
Highly expressed in brain, thymus and testis. -
Sequence similarities
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats. -
Post-translational
modificationsPhosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. -
Cellular localization
Cytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation. - Information by UniProt
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Database links
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- Unigene: 183800 Human
- Unigene: 270975 Mouse
- Unigene: 475271 Mouse
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Alternative names
- Fug 1 antibody
- Fug1 antibody
- GTPase-activating protein, RAN, 1 antibody
see all
Images
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ab2081 at a concentration of 0.2
µ g/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µ g total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. ab2081 at a concentration of 0.2µg/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µg total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. -
ab2081 (1/500) detecting RanGap1 in mitotic and interphase Hela cells (green). Dapi was used as counterstain in order to highlight the nucleus (blue) and cells were fixed both in Methanol (-20 °C/ 6 mins, followed by 3 washes in PBS) or Paraformaldehyde (3,7% PFA /10 min at room temperature, 0,1% Triton/PBS 5 min, glycine 5 min, followed by 3 washes in PBS); This antibody also gave good results in NIH-3T3 cells under both the above mentioned fixation procedures (data not shown).
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab2081 has been referenced in 3 publications.
- Zhang S et al. The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways. Nat Commun 11:3343 (2020). PubMed: 32620905
- Barbato S et al. Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability. J Cell Sci 133:N/A (2020). PubMed: 31932502
- He X et al. Characterization of the loss of SUMO pathway function on cancer cells and tumor proliferation. PLoS One 10:e0123882 (2015). PubMed: 25860128