Key features and details
- One-wash 90 minute protocol
- Sensitivity: 35 pg/ml
- Range: 0.04 ng/ml - 160 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat IgG ELISA Kit, Fluorescent
See all IgG kits
Intra-assay Sample n Mean SD CV% Rat serum 3 2.66% Inter-assay Sample n Mean SD CV% Rat serum 3 3.61%
Sample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range0.04 ng/ml - 160 ng/ml
Sample specific recovery Sample type Average % Range Antibody Diluent 4B 103.7 99.4% - 110.97% 10F DMEM Medium 79.1 67.5% - 98% OF DMEM Medium 76.5 62.8% - 94%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Rat
Does not react with: Goat, Cow, Pig
Immunoglobulin G (IgG) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Immunoglobulin G (IgG) protein in rat serum, plasma, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat serum and plasma samples contain about 7 to 10 mg/ml of IgG.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Rat IgG Capture Antibody 1 x 600µl 10X Rat IgG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 4B 1 x 6ml Plate Seals 1 unit Rat IgG Lyophilized Purified Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The Immunoglobulin G (IgG) standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated rat IgG value are graphed.
ab229388 has not yet been referenced specifically in any publications.