• Product name

  • Detection method

  • Precision

    Sample n Mean SD CV%
    overall < 10%
    Sample n Mean SD CV%
    overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 90 pg/ml
  • Range

    93.75 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 100 91% - 108%
    Plasma 101.6 84% - 114%
    Cell culture media 130.4 122% - 138%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s PDGF-AA Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat PDGF-AA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat PDGF-AA coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat PDGF-AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    280X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat PDGF-AA 2 vials
    PDGF-AA Microplate (12 x 8 wells) 1 unit
    Recombinant Rat PDGF-AA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Subunit structure: Homodimer (AA); antiparallel disulfide-linked dimer. Heterodimer with PDGFB (AB); antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4.
  • Alternative names

    • PDGF A
    • PDGF A chain
    • PDGF subunit A
    • PDGF1
    • platelet derived growth factor A chain
    • platelet derived growth factor alpha chain
    • platelet derived growth factor alpha isoform 2 preproprotein
    • platelet derived growth factor subunit A
    see all
  • Database links


Our Abpromise guarantee covers the use of ab155464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab155464 -  Assay Diluent A.

  • Representative standard curve using ab155464 - Assay Diluent B.



This product has been referenced in:

  • Li X  et al. Comparison of subacute and chronic scar tissues after complete spinal cord transection. Exp Neurol 306:132-137 (2018). Read more (PubMed: 29753649) »
See 1 Publication for this product

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