• Product name
    Rat TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    A 12 543.5pg/ml 44.7 8.2%
    B 10 130.1pg/ml 10.3 7.9%
    Sample n Mean SD CV%
    A 9 541.4pg/ml 33.2 6.1%
    B 9 149.4pg/ml 9.8 6.5%
  • Sample type
    Cell culture supernatant, Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.

    A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

    This kit will recognize both endogenous and recombinant rat TNF alpha

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-TNF alpha Red 1 x 400µl 2 x 400µl
    TMB Substrate 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    TNF alpha Microplate (12 x 8 well strips) 1 unit 2 units
    TNF alpha Standard (Lyophilized) Yellow 2 vials 4 vials
  • Research areas
  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab46070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA ((PMA) ab120297) or PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (LPS+PMA) for the last 6 hours (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab46070



This product has been referenced in:
  • Jiang P  et al. Astragaloside IV Prevents Obesity-Associated Hypertension by Improving Pro-Inflammatory Reaction and Leptin Resistance. Mol Cells 41:244-255 (2018). Read more (PubMed: 29562733) »
  • Gu R  et al. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis. Front Pharmacol 9:331 (2018). Read more (PubMed: 29681857) »

See all 18 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I look forward to receiving the resuls and order number for the ab100786 VEGF rat ELISA kit.

For the TNF kit ab46070, I think it is likely to be the sample that is the problem.However, I would be happy to...

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Thank you for your message. I look forward to receiving the further information regarding ab100786 VEGF Rat ELISA Kitso we can investigate this further for you.

I am sorry to hear you also have some concerns regarding two other kits. I would...

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Thank you for contacting us.

I have been in contact with the production group regarding your inquiry. Unfortunately, at this time, we do not offer individual components of this kit for sale. Information about capture antibody used to coat the...

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Vielen Dank für Ihre Anfrage.

Wie versprochen habe ich mich mit dem Hersteller und unserer Produktabteilung in Verbindung gesetzt, um zu erfahren, ob es das Streptavidin-HRP separat erhältlich gibt. Leider haben wir diese Einzelkomponente vo...

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Thank you for contacting us. The limit of detection of the TNF alpha ELISA kit ab46070 is 15 pg/ml, which is arrived at by taking the mean optical density of 64 blank wells (no TNF alpha), adding two standard deviations of the mean, then calculating...

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Abreview for TNF alpha Rat ELISA Kit

Good Good 4/5 (Ease of Use)
Sample: Rat Serum (rat serum)
Specification: rat serum
Amount of Sample: 0.1 µg
Type of Kit Used: ELISA
Positive control: Serial dilution of target
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Concentration estimated as serum was used.

Mrs. Rhoda Cortes

Verified customer

Submitted Aug 30 2007


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