For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    reactive-oxygen-species-ros-detection-assay-kit-ab287839.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Share by email

Reactive Oxygen Species (ROS) Detection Assay Kit (ab287839)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Reactive Oxygen Species (ROS) Detection Assay Kit Oxidative Stress Analysis
  • Reactive Oxygen Species (ROS) Detection Assay Kit Oxidative Stress Analysis 2
  • Reactive Oxygen Species (ROS) Detection Assay Kit Fluorescence Microscopy

Overview

  • Product name

    Reactive Oxygen Species (ROS) Detection Assay Kit
  • Detection method

    Flow cytometry-fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells. Low levels of ROS play an essential role in signaling pathways, whereas increased levels under oxidative stress, results in damage to nucleic acids, proteins and membrane lipids. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. The ROS Detection Assay Kit ab287839 is designed for the detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as control for the measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy.

  • Tested applications

    Suitable for: FM, Functional Studiesmore details
  • Platform

    Flow cytometer, Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests 250 tests
    ROS Assay buffer WM/NM 1 x 25ml 1 x 65ml
    ROS Inducer (250X) Yellow Cap 1 x 20µl 1 x 20µl
    ROS Label (1000X) Green Cap 1 x 10µl 1 x 25µl

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab287839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.
Notes
FM
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.

Images

  • Reactive Oxygen Species (ROS) Detection Assay Kit Oxidative Stress Analysis
    Reactive Oxygen Species (ROS) Detection Assay Kit Oxidative Stress Analysis

    Live cells labeled and treated with ROS Inducer and 100 µM of H2O2 according to the kit protocol.

    2 x104 Jurkat cells per condition were analyzed by FACS. Plotted intensity values show a significant increase in ROS production between treatments and controls

  • Reactive Oxygen Species (ROS) Detection Assay Kit Oxidative Stress Analysis 2
    Reactive Oxygen Species (ROS) Detection Assay Kit Oxidative Stress Analysis 2

    Live cells labeled and treated with ROS Inducer and 100µM of H2O2 according to the kit protocol.

    1x105 Jurkat cells were analyzed on a plate reader. Mean+/- standard deviation plotted for 3 replicates per condition

  • Reactive Oxygen Species (ROS) Detection Assay Kit Fluorescence Microscopy
    Reactive Oxygen Species (ROS) Detection Assay Kit Fluorescence Microscopy

    Profiling of ROS formation by Fluorescence Microscopy in Jurkat and MCF-7 live cells.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab287839? Please let us know so that we can cite the reference in this datasheet.

ab287839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab287839.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.