Question (17955) | Recombinant Calreticulin protein (ab15729)

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Question

>Regarding ab15729, could you please provide the exact sequence >inforamtion on the calrecticulin (and tags) used. I've read the >reference (Wen-Fang Cheng et al) and found that it is a rabbit >calcrecticulin. The customer insists that Abcam provides/confirms >the exact sequences of the recombinant protein+tag that was used in >the production ab15729. > >As the customer's experiments are dependent on this data, could you >please be so kind to provide this information.

Answer

Dear Dr. Hill, Thank you for your patience. Below is the amino acid sequence for ab15729. This rabbit calreticulin protein carries a myc-tag and his-tag at the C-terminus. I hope this information helps with the Mass Spectroscopy experiments. E P V V Y F K E Q F L D G D G W T E R W I E S K H K S D F G K F V L S S G K F Y G D Q E K D K G L Q T S Q D A R F Y A L S A R F E P F S N K G Q P L V V Q F T V K H E Q N I D C G G G Y V K L F P A G L D Q K D M H G D S E Y N I M F G P D I C G P G T K K V H V I F N Y K G K N V L I N K D I R C K D D E F T H L Y T L I V R P D N T Y E V K I D N S Q V E S G S L E D D W D F L P P K K I K D P D A S K P E D W D E R A K I D D P T D S K P E D W D K P E H I P D P D A K K P E D W D E E M D G E W E P P V I Q N P E Y K G E W K P R Q I D N P D Y K G T W I H P E I D N P E Y S P D A N I Y A Y D S F A V L G L D L W Q V K S G T I F D N F L I T N D E A Y A E E F G N E T W G V T K T A E K Q M K D K Q D E E Q R L K E E E E E K K R K E E E E A E E D E E D K D D K E D E D E D E E D K D E E E E E A A A G Q A K D E L P L E Q K L I S E E D L N S A V D H H H H H H

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